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Invitation to
The 27th A-IMBN(Asia-Pacific International Molecular Biology Network)-AMBO (Asian Molecular Biology Organization) International Training Course
''Molecular and Cell Biology in Post-Genome Era''

February 11-20, 2004
Research Institute for Microbial Diseases, Genome Information Research Center, Graduate School of Medicine and Graduate School of Frontier Biosciences, Osaka University

To whom it may concern,

Please find enclosed materials concerning the A-IMBN-AMBO international Training Course and display the poster on your information board.
The 2004 A-IMBN-AMBO International Training Course (formally called as AMBO Training Course) is organized by Dr. Akio Sugino and will be held at Research Institute for Microbial Diseases, Genome Information Research Center, Graduate School of Medicine and Graduate School of Frontier Biosciences, Osaka University from February 11 to 20, 2004. The course is supported by the Asia-Pacific International Molecular Biology Network (A-IMBN), the Protein Research Foundation, and Grant-in-Aid for The 21 Century COE programs from The Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. We aim at providing young Asian scientists with basic molecular and cell biological methodology which is useful for Life Sciences.
The training course consists of lectures and experimental courses, which will be conducted by English. The applicants should select one of the five experimental courses listed below. Each course accommodates four participants. The organizing committee will select 20 participants from the applicants considering the information given by the application form and their countries. All participants will receive a fellowship, which covers round-trip airfare, domestic transportation fee and lodging accommodations during the course.
For application, please complete the following forms and send them to the following address by October 31, 2003.
(1) The completed application forms (Forms 1 and 2)  (136k)
(2) A curriculum vitae
(3) A list of scientific publications, if any.
(4) Two letters of recommendation.

Mailing Address:
Prof. Akio Sugino
The 27th A-IMBN-AMBO International Training Course
Graduate School of Frontier Biosciences, Osaka University
3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
E-mail: asugino@biken.osaka-u.ac.jp

Laboratory Courses:

Course 1. Manipulating the Mouse Embryos and Gene Targeting
1)  General Methodology
2) Germ Cells and Embryos (basic physiology of germ cells and embryos)
3) In vitro Fertilization (handling of germ cells and observation of fertilized eggs)
4) Cryopreservation of Embryos (method to preserve the embryos in liquid nitrogen)
5) Transgenic Animals (microinjection of transgenes to pronuclei of fertilized eggs)
6) Embryonic Stem Cells and Gene Targeting (transfection and selection of ES cells)
Course directors: Prof. Masaru Okabe and Dr. Masahito Ikawa (Genome Information Research Center)

Course 2. Mouse Embryology and Cell Biology
1)  Manipulation of mouse embryos
2) Examination of gene expression in mouse embryos
3) Differentiation of embryonal stem cells
Course director: Prof. Hiroshi Hamada (Graduate School of Frontier Biosciences)

Course 3. Molecular Cell Biology
1)  Preparation of high efficiency competent E. coli cells
2) Plasmid construction to make fusion proteins
3) Expression in E. coli and yeast
4) Affinity purification of GST-fusion proteins
5) Subcellular localization of GFP-fusion proteins
6) GST-pulldown experiments and immunoblotting
Course directors: Prof. Hiroshi Nojima and Drs. Norikazu Yabuta, Daisuke Okuzaki, Takahiro Tougan, and Shinya Kimura (Department of Molecular Genetics, Research Institute for Microbial Diseases)

Course 4. Proteomics and Functional Glycomics
1)  Two-dimentional electrophoresis and Mass Spectrometry
2) Lectin blotting and lectin affinity chromatography
3) Glycosyl transferase assay
4) Pyridylamination and N-glycan analysis
Course directors: Prof. Naoyuki Taniguchi and Dr. Koichi Honke (Graduate School of Medicine)

Course 5. Yeast Molecular Genetics
1)  Yeast transformation, gene replacement, and Southern Blot analysis including PCR analysis
2) Two hybrid analysis
3) Protein extraction and Western analysis
4) Chromosome spreading analysis (visualization of proteins bound on yeast chromatin)
5) Mutagenesis and plasmid shuffling
6) Cell cycle and FASC analysis
7) DNA tip analysis
Course directors: Prof. Akio Sugino and Dr. Yasuo Kawasaki (Graduate School of Frontier Biosciences)



An Outline of The 27th A-IMBN-AMBO International Training Course


Lecturers (tentative)
Prof. Mitsuhiro Yanagida (Kyoto Univ.)
Prof. Kunihiro Matsumoto (Nagoya Univ.)
Prof. Tohro Nakano (Osaka Univ.)
Prof. Katsuro Koike (Cancer Res. Ins.)
Prof. Haruki Nakamura (Osaka Univ.)
Prof. Ken-Ichi Arai (Univ. of Tokyo)
Prof. Toshio Yanagida (Osaka Univ.)
Prof. Taro Kinoshita (Osaka Univ.)
Prof. Hisao Masai (Tokyo Metro. Inst.)
 

Laboratory Courses
1. Manipulating the mouse Embryos and Gene targetting (Prof. Masaru Okabe)
2. Mouse Embryology and Cell Biology (Prof. Hiroshi Hamada)
3. Molecular Cell Biology (Prof. Hiroshi Nojima)
4. Proteomics and Functional Glycomics (Prof. Naoyuki Taniguchi)
5. Yeast Molecular Genetics (Prof. Akio Sugino)

The training course consists of lectures and laboratory courses, and the trainees select one of the laboratory course.
Organized by Prof. Akio Sugino (Graduate School of Frontier Biosciences, Osaka University)
Supported by the Asia-Pacific International Molecular Biology Network (A-IMBN), The Protein Research Foundation, and Grant-in-Aid for The 21 Century COE programs-''Integrated Functional Analyses of Disease-Associated Sugar Chains and Proteins''& ''Dynamics of Life Systems'' from The Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan


Applications should arrive by October 31, 2003 to Dr. Akio Sugino,Graduate School of Frontier Biosciences,Osaka University,3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
Tel: 81-6-6879-8331, Fax: 81-6-6877-3584
E-mail: asugino@biken.osaka-u.ac.jp


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Integrated functional analyses of disease-associated sugar chains and proteins, Osaka University