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Invitation
to
The 27th A-IMBN(Asia-Pacific
International Molecular Biology Network)-AMBO
(Asian Molecular Biology Organization)
International Training Course
''Molecular and
Cell Biology in Post-Genome Era''
February 11-20, 2004
Research Institute for Microbial Diseases,
Genome Information Research Center,
Graduate School of Medicine and Graduate
School of Frontier Biosciences, Osaka
University |
To whom
it may concern,
Please find enclosed
materials concerning the A-IMBN-AMBO
international Training Course and display
the poster on your information board.
The 2004 A-IMBN-AMBO
International Training Course (formally
called as AMBO Training Course) is organized
by Dr. Akio Sugino and will be held
at Research Institute for Microbial
Diseases, Genome Information Research
Center, Graduate School of Medicine
and Graduate School of Frontier Biosciences,
Osaka University from February 11 to
20, 2004. The course is supported by
the Asia-Pacific International Molecular
Biology Network (A-IMBN), the Protein
Research Foundation, and Grant-in-Aid
for The 21 Century COE programs from
The Ministry of Education, Culture,
Sports, Science and Technology (MEXT)
of Japan. We aim at providing young
Asian scientists with basic molecular
and cell biological methodology which
is useful for Life Sciences.
The training course consists
of lectures and experimental courses,
which will be conducted by English.
The applicants should select one of
the five experimental courses listed
below. Each course accommodates four
participants. The organizing committee
will select 20 participants from the
applicants considering the information
given by the application form and their
countries. All participants will receive
a fellowship, which covers round-trip
airfare, domestic transportation fee
and lodging accommodations during the
course.
For application, please
complete the following forms and send
them to the following address by October
31, 2003.
(1) The
completed application forms (Forms 1
and 2) (136k)
(2) A curriculum vitae
(3) A list of scientific publications,
if any.
(4) Two letters of recommendation.
Mailing
Address:
Prof. Akio Sugino
The 27th A-IMBN-AMBO International
Training Course
Graduate School of Frontier Biosciences,
Osaka University
3-1 Yamada-oka, Suita, Osaka 565-0871,
Japan
E-mail: asugino@biken.osaka-u.ac.jp |
Laboratory Courses:
Course
1. Manipulating the Mouse Embryos
and Gene Targeting |
1)
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General
Methodology |
2)
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Germ Cells and
Embryos (basic physiology of germ
cells and embryos) |
3)
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In vitro Fertilization
(handling of germ cells and observation
of fertilized eggs) |
4) |
Cryopreservation
of Embryos (method to preserve
the embryos in liquid nitrogen) |
5) |
Transgenic Animals
(microinjection of transgenes
to pronuclei of fertilized eggs) |
6)
|
Embryonic Stem
Cells and Gene Targeting (transfection
and selection of ES cells) |
Course
directors: Prof. Masaru Okabe
and Dr. Masahito Ikawa (Genome
Information Research Center) |
Course
2. Mouse Embryology and Cell Biology |
1)
|
Manipulation
of mouse embryos |
2)
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Examination of
gene expression in mouse embryos |
3)
|
Differentiation
of embryonal stem cells |
Course
director: Prof. Hiroshi Hamada
(Graduate School of Frontier Biosciences) |
Course
3. Molecular Cell Biology |
1)
|
Preparation
of high efficiency competent E.
coli cells |
2)
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Plasmid construction
to make fusion proteins |
3)
|
Expression in
E. coli and yeast |
4) |
Affinity purification
of GST-fusion proteins |
5) |
Subcellular localization
of GFP-fusion proteins |
6)
|
GST-pulldown
experiments and immunoblotting |
Course
directors: Prof. Hiroshi Nojima
and Drs. Norikazu Yabuta, Daisuke
Okuzaki, Takahiro Tougan, and
Shinya Kimura (Department of Molecular
Genetics, Research Institute for
Microbial Diseases) |
Course
4. Proteomics and Functional Glycomics |
1)
|
Two-dimentional
electrophoresis and Mass Spectrometry |
2)
|
Lectin blotting
and lectin affinity chromatography |
3)
|
Glycosyl transferase
assay |
4) |
Pyridylamination
and N-glycan analysis |
Course
directors: Prof. Naoyuki Taniguchi
and Dr. Koichi Honke (Graduate
School of Medicine) |
Course
5. Yeast Molecular Genetics |
1)
|
Yeast
transformation, gene replacement,
and Southern Blot analysis including
PCR analysis |
2)
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Two hybrid analysis |
3)
|
Protein extraction
and Western analysis |
4) |
Chromosome spreading
analysis (visualization of proteins
bound on yeast chromatin) |
5) |
Mutagenesis and
plasmid shuffling |
6)
|
Cell cycle and
FASC analysis |
7) |
DNA tip analysis |
Course
directors: Prof. Akio Sugino and
Dr. Yasuo Kawasaki (Graduate School
of Frontier Biosciences) |
|
An Outline of The 27th A-IMBN-AMBO
International Training Course
Lecturers (tentative)
Prof. Mitsuhiro
Yanagida (Kyoto Univ.) |
Prof. Kunihiro
Matsumoto (Nagoya Univ.) |
Prof. Tohro Nakano
(Osaka Univ.) |
Prof. Katsuro
Koike (Cancer Res. Ins.) |
Prof. Haruki Nakamura
(Osaka Univ.) |
|
Prof. Ken-Ichi
Arai (Univ. of Tokyo) |
Prof. Toshio Yanagida
(Osaka Univ.) |
Prof. Taro Kinoshita
(Osaka Univ.) |
Prof. Hisao Masai
(Tokyo Metro. Inst.) |
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Laboratory
Courses |
1. |
Manipulating the mouse
Embryos and Gene targetting (Prof. Masaru
Okabe) |
2. |
Mouse Embryology and
Cell Biology (Prof. Hiroshi Hamada) |
3. |
Molecular Cell Biology
(Prof. Hiroshi Nojima) |
4. |
Proteomics and Functional
Glycomics (Prof. Naoyuki Taniguchi) |
5. |
Yeast Molecular Genetics
(Prof. Akio Sugino) |
The training course consists of lectures
and laboratory courses, and the trainees select
one of the laboratory course.
Organized by Prof. Akio Sugino (Graduate School
of Frontier Biosciences, Osaka University)
Supported by the Asia-Pacific International
Molecular Biology Network (A-IMBN), The Protein
Research Foundation, and Grant-in-Aid for
The 21 Century COE programs-''Integrated Functional
Analyses of Disease-Associated Sugar Chains
and Proteins''& ''Dynamics of Life Systems''
from The Ministry of Education, Culture, Sports,
Science and Technology (MEXT) of Japan
Applications should
arrive by October 31, 2003 to Dr. Akio
Sugino,Graduate School of Frontier Biosciences,Osaka
University,3-1 Yamada-oka, Suita, Osaka
565-0871, Japan
Tel: 81-6-6879-8331, Fax: 81-6-6877-3584
E-mail: asugino@biken.osaka-u.ac.jp |
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Copyright 2003 Osaka University. All Right Reserved.
Integrated functional analyses of disease-associated sugar chains and proteins, Osaka University
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